Sevilla will be looking for their first points in Group H when they host Slavia Prague on Tuesday night.
The Spanish side, who enjoyed back-to-back
UEFA Cup successes prior to this season, were beaten 3-0 by Arsenal on matchday one.
Sevilla and Slavia had contrasting results at the weekend, with the latter thrashing FC Zlin 7-1 in Czech Liga 1 while Sevilla went down 2-0 against Real Zaragoza, their third consecutive La Liga defeat.
Sevilla coach Juande Ramos was bullish following the Zaragoza defeat, saying: "We lost but we were infinitely superior. The way things worked out and the final result was very surprising for me.
"There isn't a logical explanation for what happened. There are few reasons I can give you, only that we weren't assertive enough up front."
Sevilla have been boasted by the return to fitness of Frederic Kanoute and Luis Fabiano this week. Both should be fit to face the Czech side.
Ramos added: "We're competing in a competition that is much more demanding than what we have encountered in the past.
"It's such a short competition that you have to get wins. It's vital that we get three points so we can pull level with Slavia and keep our hopes up.
"Getting a win will help us get over this worry we have had recently because this is not a situation Sevilla has recently been used to. We are all uncomfortable. It's vital because we don't want to be a long way off our opponents."
Ramos rates Tuesday's opponents from the Czech Republic, saying: "Slavia are tough, very competitive - not full of stars but of accomplished footballers. If they won 7-1 at the weekend and eliminated Ajax in the qualifiers it won't be easy for us.
"They are a typical eastern team - tough, competitive and fast."
Winger Jesus Navas told
.: Sevilla Fútbol Club, S.A.D. :. "We will have to impose a fast rhythm if we are to win. We are working hard to try and reverse the trend and this is a good chance to do that."
Slavia will be looking to build on their 2-1 opening game victory against Steaua Bucharest and 7-1 thrashing of FC Zlin.
Coach Karel Jarolim does not want his side getting carried away, though.
After the weekend victory he said: "I don't want to dwell on this result. The margin is not important as it only brought us three points, just like a 1-0 victory. But I am satisfied with the style of our play.
"We scored two early goals and from then on it was a one-sided affair. My only regret is that we conceded."
Slavia will be without Vladimir Smicer (thigh), Frantisek Doizdal (sprained ankle), Martin Latka (thigh) and Erich Brabec (knee).
Michal Svec returned at the weekend from a broken toe and looks set to feature.
Slavia are making their Champions League debut, having been eliminated at the third qualifying stage four times in the last seven years before this season's sensational win over Ajax.